Steven D. Schwartzbach

Steven D. Schwartzbach

Professor

Phone
901.678.4470
Fax
901.678.4457
Office
Life Sciences 423
Office Hours
By Appointment

About Dr. Schwartzbach

Dr. Schwartzbach came to the Department in 2001 from the University of Nebraska. He teaches Biology 3130 Cell Biology. He began his research career characterizing the coding capacity of the chloroplast genome and established his laboratory in Nebraska focusing on the photoregulation of chloroplast development in the protist Euglena. In the course of these studies he found that some Euglena proteins were synthesized as polyprotein precursors, that Euglena mRNAs contained a common trans-spliced 5' end and that Euglena transcripts did not contain the canonical intron exon junctions characteristic of most eukaryotic messages. His current research focuses on the unique ER to Golgi to chloroplast transport pathway for cytoplasmically synthesized chloroplast proteins and the process of secondary endosymbiosis responsible for the evolution of complex plastids; plastids surrounded by three or four membranes rather than the two membranes of higher plants and most algae.

Research Interests

• Biochemistry of protein targeting and import into secondary plastids
• Molecular mechanism of chloroplast genome reduction and loss
• Chloroplast evolution

Education

B.S. Biology, State University of New York at Buffalo; Ph.D. Biology, Brandeis University; American Cancer Society Post-Doctoral Fellow, Oak Ridge National Laboratory.

Recent Publications

  • Vaidyanathan, H., You Zhou, T. M. Petro and S. D. Schwartzbach, 2003. Intracellular localization of the p35 subunit of murine IL-12. Cytokine 21: 120-128
  • Sláviková, S., Vacula, R., Fang, Z., Ehara, T., Osafune, T., Schwartzbach, S.D. 2005. Homologous and heterologous reconstitution of Golgi to chloroplast transport and protein import into the complex chloroplasts of Euglena. J. Cell Sci 118: 1651-1661
  • Vacula, R., Sláviková, S., Schwartzbach, S.D. 2007. Protein trafficking to the complex chloroplasts of Euglena. (Invited chapter): Protein Targeting Protocols: Second edition. Methods in Molecular Biology, Vol. 390. (M. van der Giezen ed.) Humana Press, Totowa, NJ. pp. 219-237
  • Osafune, T. and Schwartzbach, S.D. 2007. Intracellular protein localization by immunoelectron microscopy. (Invited chapter) Protein Targeting Protocols: Second edition. Methods in Molecular Biology, Vol. 390. (M. van der Giezen ed.) Humana Press, Totowa, NJ. pp. 407-416
  • Osafune, T. and Schwartzbach, S.D. 2010. Immunoelectron microscopy of chemically fixed developing plant embryos. (Invited chapter) Immunoelectron Microscopy: Methods and Protocols. Methods in Molecular Biology, Vol. 657. (S.D. Schwartzbach and T. Osafune eds.) Humana Press, New York, NY. pp. 123-132
  • Osafune, T. and Schwartzbach, S.D. 2010. Serial section immunoelectron microscopy of algal cells. (Invited chapter) Immunoelectron Microscopy: Methods and Protocols. Methods in Molecular Biology, Vol. 657. (S.D. Schwartzbach and T. Osafune eds.) Humana Press, New York, NY. pp. 259-274
  • Schwartzbach, S.D. and Osafune, T. 2010 Immunoelectron Microscopy: Methods and Protocols: Methods in Molecular Biology, Vol. 657. Humana Press, New York, NY. 352 pp.
  • Ehara, T., Nara, M., Schwartzbach, S. D. and Osafune, T. Tomoko. 2011. A scanning electron microscopic method for the study of bacterial growth inhibition by the paper disc method. Cytologia 76: 49-54
  • Nyindodo-Ogari, L., Schwartzbach, S and C. Estrano 2014. Giardia mitosomal protein import machinery differentially recognizes mitochondrial targeting signals. Infectious disorders-drug targets 14: 23-29
  • Krajcovic, J., Vesteg, M., Schwartzbach, S.D. 2015. Euglenoid flagellates: A multifaceted biotechnology platform. J. Biotechnol. 202: 135-145
  • Nyindodo-Ogari, L., Schwartzbach, SD,Skalli, O., Estrano CE. 2016 Localizing proteins in fixed Giardia lamblia and live cultured cells by confocal microscopy. (Invited chapter) High-Resolution Imaging of Cellular Proteins: Methods and Protocols. Methods in Molecular Biology, Vol. 1474. (S.D. Schwartzbach, O. Skalli and T. Schikorski eds.) Humana Press, New York, NY. pp. 93-111
  • Hadariová, L, Vesteg, M, Bircák, E, Schwartzbach, SD, Krajcovic, J, 2016. An intact plastid genome is essential for the survival of colorless Euglena longa but not Euglena graclis. Curr. Genet doi:10.1007/s00294-016-0641-z
  • Schwartzbach, S.D. 2017. Photo and Nutritional Regulation of Euglena Organelle Development. (Invited chapter) Euglena: Biochemistry, Cell and Molecular Biology. Advances in Experimental Medicine and Biology, Vol. 979. (S. D. Schwartzbach and S. Shigeoka eds) Springer International In press
  • Durnford, D. and Schwartzbach, S. D. Protein targeting to Euglena chloroplasts. (Invited chapter) Euglena: Biochemistry, Cell and Molecular Biology. Advances in Experimental Medicine and Biology, Vol. 979. (S. D. Schwartzbach and S. Shigeoka eds) Springer International In press