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Purpose of the Center

The purpose of the W. Harry Feinstone Center for Genomic Research is to provide research and graduate education in the area of genetic research, particularly functional genomics, while providing a focal point for this activity within the University of Memphis and the Memphis Scientific community.

Support of Research Infrastructure

The W. Harry Feinstone Center for Genomic Research has developed and maintains a Biology Instrumentation Core (BIC), we provide DNA Microarray technologies as well as NGS DNA sequencing and data analysis.

In 2002, we added an Affymetrix GeneChip system for high-throughput DNA microarray analysis. In 2013, the BIC acquired the Illumina MiSeq benchtop sequencer, further advancing the center’s functional genomics focus. Today the center is capable of sequencing reference genomes for many scientifically important species. During the past year, the core has undertaken the task of sequencing the entire genome of the Tennessee Ladybug (Coccinella septempunctata) using the Illumina MiSeq sequencer.

The Center utilizes the HPC computational resources provided by ITS at the Univefluorescent_microscopersity of Memphis as well as an FCGR dedicated computer cluster.

The BSIC also provide case-by-case consultative services for DNA microarray, next generation DNA sequencing, DNA size selection, cloning and protein expression, realtime PCR, tissue culture and cell transfection. This capability has been made possible by the expertise brought to the Center by Drs. Thomas and Carrie Sutter, as well as the capitalization of equipment that has occurred over twelve years since the Center’s development.

 

The Center provides salary support for the BIC, as well as maintenance of instrumentation service contracts. To date, the center has purchased over million dollars in advanced molecular biology instrumentation and maintains the equipment by supporting service contracts. In addition to these center-supported facilities, the Sutter Lab and the Biology Department have established common equipment facilities (LS543) available to Biology faculty and other center members.

These facilities include following equipment: Agilent Bioanalyzer Chip-on-chip technology, Covaris Ultra Sonicater, Bio Rad Molecular Imager FX (Phosphor Imager with laser upgrades), Bio Rad iCycler with optical upgrade for RealTime PCR, MilliQ water system, Sage Blue Pippin DNA size selection system, Thermofisher Scientific NanoDrop, Invitrogen Qubit Fluorometer, Ultra and high speed centrifuges and rotors, Beckman DU640 Spectrophotometer, 2 Speed-Vacuums and a gel dryer, Bio Rad Gel Documentation System with computer and a Cary Eclipse Fluorescence Spectrophotometer.

 

Student Support

The Center has provided stipends for eight graduate students conducting research in the Department of Biology, Chemistry and the Computer Science Division of the Department of Mathematical Sciences (BioInformatics). Also, the Center has supported two undergraduate research internships, one in biology and the other through the NSF-supported REU program of the Department of Chemistry. The Center has also supported undergraduate internships.

Interdisciplinary and Inter-institutional Research and Education

One of the primary goals of the Center for Genomic Research is to develop an Interdisciplinary Research Program. The driving force behind such a program is to leverage the Center’s resources to increase the research and training environment of the University of Memphis. By emphasizing interdisciplinary research, it is hoped that the Center can aid in the development and strengthening of University of Memphis educational programs that in turn support graduate research.

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Last Updated: 7/28/14