Users call this instrument the “confocal microscope”. It should be understood, however,
that this microscope is build around a “regular microscope” or more appropriately
a bright-field light microscope, the Nikon Ti-E, onto which components are added that
enable two other modalities of microscopy: wide-field fluorescence microscopy and
confocal scanning fluorescence microscopy.
Click here to learn more about the definition of these different modalities of light
The “confocal microscope” can be used for live cell imaging applications (fluorescent
and non-fluorescent), acquisition of high quality confocal fluorescent images with
up to four fluorochromes, rapid z-stack acquisition of confocal images for 3D reconstruction
of objects, photobleaching, FRET, and spectral deconvolution of fluorophores with
overlapping emission spectra.
Users interested in quickly evaluating the results of their immunofluorescence experiments
(did the staining work or not?) can do so using the Nikon A1 in wide-field fluorescence
mode, but should consider that it may be more practical and faster to use the Nikon Eclipse 800 for that purpose. Similarly, observations of tissue sections stained by immunoperoxidase
or standard histology dyes such as H&E can be performed with the Nikon A1 in bright-field
mode, but the Nikon Eclipse 800 is more practical for that purpose and will provide
very good pictures.
Finally, the Nikon A1 cannot be used for specimens requiring an upright microscope.
These specimens may be examined with the Nikon Eclipse 800, which also enables bright-field,
Click Here for Technical Specifications