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NIKON A1 SPECIFICATIONS

Microscope Base

  • Ti-E inverted microscope

  • 10x eyepieces

  • Objectives:

    • 10x Plan Fluor DLL, NA 0.3, WD 16 mm

    • 20x Super plan fluor, NA 0.45, WD 8.2-6.9 mm, Ph1

    • 20x Plan apo, violet corrected, NA O.75, WD 1mm

    • 40x Plan fluor, oil, NA 1.30, WD 0.2 mm

    • 60x Plan apo, violet corrected, oil, NA 1.4m WD 0.13 mm

  • DIC components:

    • DIC sliders 60x, 40x, 20x,

    • DIC modules LWD N2 dry, LWD N1 dry

  • Perfect focus system with motorized nosepiece

  • Motorized stage with encoder and controller

  • Slide and 60 mm Petri dish stage holders

  • Transmitted light detector

  • Diascopic illumination (12V 100 W halogen lamp and 45 mm heat absorbing filter)

 

Wide-field Fluorescence Microscopy Components

  • Lumen 200 illumination system

  • Motorized turret for the filter sets:

    • DAPI filter set:

      • Excitation filter: FF01-387/11-25

      • Emission filter: FF01-447/60-25

      • Dichroic beam splitter: FF409-Di02-25x36

    • GFP filter set:

      • Excitation filter: FF01-472/30-25

      • Emission filter: FF01-520/35-25

      • Dichroic beam splitter: FF495-Di02-25x36

    • TRITC filter set:

      • Excitation filter: FF01-543/22-25

      • Emission filter: FF01-593/40-25

      • Dichroic beam splitter: FF562-Di02-25x36

    • CY5 filter set:

      • Excitation filter: FF01-628/40-25

      • Emission filter: FF01-692/40-25

      • Dichroic beam splitter: FF660-Di0-25x36

 

Confocal Fluorescence Scanning Microscopy Components

  • Lasers:

    • 405 nm, coherent diode laser, 100 mw

    • 488 nm, coherent DPSS diode laser, 50 mw

    • 561 nm, DPSS laser, 50 mw

    • 640 nm, coherent diode laser, 40 mw

  • Scanning heads:

    • Scanning head for standard image acquisition (non-resonant scanner; pixel size: max 4096x4096 pixels; scanning speed: 4 fps at 512x512); zoom: 1-1000x continuous; scanning modes: X-Y, XY rotation, free line, line z.

    • Scanning head for high-speed image acquisition (resonant scanner on x-axis with 7.8 Hz resonance frequency; non-resonant scanner on y-axis; pixel size max 512x512 pixels; scanning speed: 30 fps (512x512) to 420 fps (512x32 pixels); 15,600 lines/sec; zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x); scanning mode: X-Y, line

  • Detectors:

    • Standard mode with 4 PMTs

    • Spectral detection with 32 channels, 2.5 nm, 6 nm and 10 nm resolution

 

Stage Top Incubator

  • Tokai Hit INUG2A-TIZ incubator with heater and gas mixer

  • Tokai Hit 35 mm Petri dish holder

 

Imaging Workstation and Software Components

  • 64 bits workstation with Intel Core i7 SIPP 2.8 GHz processor

  • 16 GB DDR3 RAM

  • 1 TB hard drive

  • 20 inches wide format active matrix TFT LCD monitor

  • NIS-Elements C imaging software for A1 confocal applications

  • NIS-Elements Hardware control module

 

 

 

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Last Updated: 9/26/14