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Nikon A1 Specifications
1) Microscope Base
  • Ti-E inverted microscope
  • 10x eyepieces
  • Objectives:
    • 10x Plan Fluor DLL, NA 0.3, WD 16 mm
    • 20x Super plan fluor, NA 0.45, WD 8.2-6.9 mm, Ph1
    • 20x Plan apo, violet corrected, NA O.75, WD 1mm
    • 40x Plan fluor, oil, NA 1.30, WD 0.2 mm
    • 60x Plan apo, violet corrected, oil, NA 1.4m WD 0.13 mm
  • DIC components:
    • DIC sliders 60x, 40x, 20x,
    • DIC modules LWD N2 dry, LWD N1 dry
  • Perfect focus system with motorized nosepiece
  • Motorized stage with encoder and controller
  • Slide and 60 mm Petri dish stage holders
  • Transmitted light detector
  • Diascopic illumination (12V 100 W halogen lamp and 45 mm heat absorbing filter)

2) Wide-field Fluorescence Microscopy Components

  • Lumen 200 illumination system
  • Motorized turret for the filter sets:
    • DAPI filter set:
      • Excitation filter: FF01-387/11-25
      • Emission filter: FF01-447/60-25
      • Dichroic beamplitter: FF409-Di02-25x36
    • GFP filter set:
      • Excitation filter: FF01-472/30-25
      • Emission filter: FF01-520/35-25
      • Dichroic beamplitter: FF495-Di02-25x36
    • TRITC filter set:
      • Excitation filter: FF01-543/22-25
      • Emission filter: FF01-593/40-25
      • Dichroic beamplitter: FF562-Di02-25x36
    • CY5 filter set:
      • Excitation filter: FF01-628/40-25
      • Emission filter: FF01-692/40-25
      • Dichroic beamplitter: FF660-Di0-25x36

3) Confocal Fluorescence Scanning Microscopy Components

  • Lasers:
    • 405 nm, coherent diode laser, 100 mw
    • 488 nm, coherent DPSS diode laser, 50 mw
    • 561 nm, DPSS laser, 50 mw
    • 640 nm, coherent diode laser, 40 mw
  • Scanning heads:
    • Scanning head for standard image acquisition (non-resonant scanner; pixel size: max 4096x4096 pixels; scanning speed: 4 fps at 512x512); zoom: 1-1000x continuous; scanning modes: X-Y, XY rotation, free line, line z.
    • Scanning head for high-speed image acquisition (resonant scanner on x-axis with 7.8 Hz resonance frequency; non-resonant scanner on y-axis; pixel size max 512x512 pixels; scanning speed: 30 fps (512x512) to 420 fps (512x32 pixels); 15,600 lines/sec; zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x); scanning mode: X-Y, line
  • Detectors:
    • Standard mode with 4 PMTs
    • Spectral detection with 32 channels, 2.5 nm, 6 nm and 10 nm resolution

4) Stage Top Incubator

  • Tokai Hit INUG2A-TIZ incubator with heater and gas mixer
  • Tokai Hit 35 mm Petri dish holder

5) Imaging Workstation and Software Components

  • 64 bits workstation with Intel Core i7 SIPP 2.8 GHz processor
  • 16 GB DDR3 RAM
  • 1 TB hard drive
  • 20 inches wide format active matrix TFT LCD monitor
  • NIS-Elements C imaging software for A1 confocal applications
  • NIS-Elements Hardware control module

 

 

 

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Last Updated: 10/30/13