Dr. Skalli is a cell biologist whose research interest is the cytoskeleton, an intracellular
network of filaments and tubules central to cell motility, cell division, cell shape
determination, and organelle trafficking. The use of microscopy is central to Dr Skalli’s
research, whether it is conventional light microscopy to examine by immunoperoxidase
or immunofluorescence the distribution of specific proteins in tissues, or confocal
laser fluorescence scanning microscopy to investigate the distribution, interaction
and function of diverse cytoskeletal proteins in cultured cells. Dr. Skalli also uses
electron microscopy to address questions related to the mechanisms by which cytoskeletal
proteins assemble to form filaments and tubules. In addition to microscopy, Dr. Skalli
expertise includes tissue culture, DNA cloning and expression in cells, as well as
several biochemical methods such as Western blotting, protein purification, and immunoprecipitation.
Dr. Skalli orversees the organization and the development of the Integrated Microscopy
Center and assists users interested with projects involving cell culture methods and
light microscopy with their experimental design, data interpretation and troubleshooting.
Dr. Skalli is also Associate Professor in the Life Sciences Department, and teaches
a graduate course on “Light Microscopy: Theory and Applications”. This course exposes
students to the various modalities of light microscopy, ranging from classical bright
field microscopy to state of the art methods such as super-resolution. The course
also involves a practical component during which students are trained in histology
and immunofluorescence staining methods and in using light, fluorescent and confocal
laser scanning microscopes.
Excerpts from Dr. Skalli CV
1982: MSc. (Biology), University of Geneva, Switerland
1987: PhD (Molecular and Cellular Biology), University of Geneva, Switzerland
1988-1992: Postdoctoral Fellow, Dept. of Cell and Molecular Biology, Northwestern
University Medical School, Chicago, IL
2010 - present: Director, Integrated Microscopy Center, University of Memphis, Memphis,
2010 - present: Associate Professor, Biological Sciences, University of Memphis, Memphis,
2002-2010: Associate Professor, Cellular Biology and Anatomy, Louisiana State University
Health Sciences Center, Shreveport, LA
1996-2002: Assistant Professor, Dpt. of Anatomy and Cell Biology, University of Illinois
at Chicago Medical School, Chicago, IL
1992-1996: Res. Assistant Professor, Dpt of Cell and Molecular Biology, Northwestern
University Medical School, Chicago, IL
Publications (Past 5 years, out of a total of 66 peer-reviewed publications and 10
Tatom, J. B., Wang, D. B., Dayton, R. D., Skalli, O., Hutton, M. L., Dickson, D. W.,
and Klein, R. L. 2009. Mimicking aspects of frontotemporal lobar degeneration and
Lou Gehrig's disease in rats via TDP-43 overexpression. Molec. Therapy, 17:607-613.
Steffan, J. J., Snider, J. L., Skalli, O., Welbourne, T., and Cardelli, J. A. 2009.
Na+/H+ exchangers and RhoA regulate acidic extracellular pH-induced lysosome trafficking
in prostate cancer cells. Traffic 10:737-753.
Quincy, Q. and Skalli, O. 2010. a-Actinin 1 and a-actinin 4 in astrocytoma cells:
contrasting role in motility, common roles in antagonizing cell death and rhoA signaling.
Exp. Cell Res. 316:1137-1147.
Luna, G., Lewis, G. P., Banna, C. D., Skalli, O., and Fisher, S. K. 2010. Expression
profiles of nestin and synemin in reactive astrocytes and müller cells following retinal
injury: a comparison with GFAP and vimentin. Mol. Vis. 16: 2511-2523.
Cardelli, J. and Skalli, O. 2010. Divide and invade: the dynamic cytoskeleton of glioblastoma
cells. Chapter in: "Glioblastoma: Molecular Mechanisms of Pathogenesis and Current
Therapeutic Strategies." Editor: S. Ray. Springer (New York). pp167-183.
Quick, Q., Pan, Y., and Skalli, O. 2011. Role of Synemin in astrocytoma cell migration.
Chapter in: “Tumor of the Central Nervous System. Vol. 5: Astrocytomas, hemangioblastomas,
and ganglioblastomas”. Editor: M.A. Hayat. Springer (Dordrecht, Heidelberg, London,
New York). pp 81-88.
Pitre, A., Davis, N., Paul, M., Orr, A.W., and Skalli, O. 2012. Synemin promotes AKT-dependent
glioblastoma cell proliferation by antagonizing PP2A. Mol. Biol. Cell. 23:1243-1253.
Sabri, F., Boughter, J.D. Jr, Gerth, D., Skalli, O., Phung, T.C., Tamula, G.R., Leventis,
N. 2012. Histological evaluation of the biocompatibility of polyurea crosslinked silica
aerogel implants in a rat model: a pilot study. PLoS One 7:e50686. Epub 2012 Dec 12.
Hamill, K.J., Hopkinson, S.B., Skalli, O., Jones, J.C. 2013. Actinin-4 in keratinocytes
regulates motility via an effect on lamellipodia stability and matrix adhesions. FASEB
Pekny, T., Faiz, M., Wilhelmsson, U., Curtis, M.A., Matej, R., Skalli, O., Pekny,
M. 2013. Synemin is expressed in reactive astrocytes and Rosenthal fibers in Alexander
disease. APMIS. Apr 18. doi: 10.1111/apm.12088. [Epub ahead of print]
Skalli, O., Wilhelmsson, U., Örndahl, L., Fekete, B., Malmgren, K., Rydenhag, B.,
and Pekny, M. 2013. Astrocytoma grade IV displays three subtypes with unique expression
profiles of intermediate filament proteins. In Press, Human Pathology.
Skalli, O., Boykins, L., Coons, L. Electron microscopy. Chapter in: “Biofouling Methods”.
Wiley. In Press.