Description and Applications:
Acquired early 2000, the Nikon D-Eclipse C1 was the first confocal scanning fluorescence
microscope at the University of Memphis. While the confocal fluorescent microscopy
needs of most users are best served with the recently acquired Nikon A1 confocal microscope,
the Eclipse C1 comes in handy for users whose samples can only be imaged with an upright
microscope (click here to learn more about upright versus inverted microscopes). The
Eclipse C1 uses He-Ne lasers to illuminate the specimen. Images of the fluorescent
specimen (FITC, TRITC, and Cy5) are acquired with a set of PMTs and assembled with
the E-Z C1 software.
The Nikon Eclipse 800 is very useful to quickly examine and take pictures of fluorescent
preparations by wide-field fluorescence (DAPI, FITC, and TRITC). In 2013, the original
mercury lamp fluorescence illumination system was replaced by a Lumen 200 advanced
fluorescence illumination system. The Lumen 200 uses a metal-halide bulb with a very
long life span and features an optimized light path to maximize the illumination of
the specimen. Several objectives can be mounted on the turret of the Eclipse 800:
1x (Plan UW, NA 0.04, WD 3.2), 2x (Plan UW, NA 0.06, WD 7.5), 10x (Plan Fluor, NA
0.30, WD 16.0), 20x (Plan apo, NA O.75, WD 1mm), 40x (Plan apo, oil, NA 1.0, WD 0.16 mm), 60x (Plan apo, oil, NA 1.4m, WD 0.21 mm), 60x (Plan apo, water, NA 1.2).
The Eclipse 800 is also excellent for bright-field microscopic observations of preparations
stained by H&E (or other histological stain) and by immunohistochemistry.
Finally, epi-illumination can be performed with the Eclipse 800 for microscopic investigations
of opaque objects. Click here to learn more about epi-illumination.
Digital micrographs of specimens observed by bright field microscopy, epi-illumination
or wide-field fluorescence can be taken with a Nikon Digital Camera DXM1200F and its
associated ACT-1 software.